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The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has been noticed in lots of tumors, which ends up in chromosome instability, and thus may perhaps add to tumorigenesis. This protein was found to affiliate with, and become involved in, the phosphorylation of NPAT protein (nuclear protein mapped for the ATM locus), which participates in cell-cycle controlled histone gene expression and plays a crucial function in endorsing mobile-cycle progression while in the absence of pRB. Two alternatively spliced transcript variants of the gene, which encode unique isoforms, happen to be explained. Two added splice variants were being described but specific nucleotide sequence details isn't nonetheless obtainable.
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Blocking peptides are peptides that bind particularly to your goal antibody and block antibody binding. These peptide ordinarily includes the epitope regarded by the antibody.
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This tutorial outlines further doable optimization of cellular and immunoassay parameters to make certain the best possible results are...
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This guide outlines even further probable optimization of mobile and immunoassay parameters to make sure the absolute best outcomes are...
Antibodies bound to the blocking peptide no longer bind towards the epitope within the concentrate on protein. By evaluating the staining from the blocked antibody vs . the antibody on your own, one can see which staining is distinct.
Target Information and facts Cyclin E belongs into the very conserved cyclin spouse and children, whose customers are characterized by a spectacular periodicity in protein abundance with the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins show distinctive expression and degradation patterns which lead towards the temporal coordination of each mitotic function. Cyclin E forms a fancy with and capabilities as a regulatory subunit of CDK2, whose activity is needed for mobile cycle G1/S transition. This protein accumulates at the G1-S section boundary and is also degraded as cells development via S period.
The measurement of protein phosphorylation is a useful tool for measuring the modulation of receptor activation by equally antibodies...
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